Morphology | Xylophilus ampelinus |
CELLULAR |
Staining | Gram negative |
Morphology | straight or slightly curved rods (0.4-0.8 x 0.6-3.3 um) Filamentous cells (30 um or more) may occur in older cultures. Cells occur singly, in pairs or in short chains |
Motility | single polar flagellum |
Specialized structures |
Division |
COLONIAL |
Solid surface | Colonies on nutrient agar are circular, semitranslucent, slightly raised, glistening and pale yellow eith entire margins. After 6 days the colonies are 0.2-0.3 mm in diameter. After 15 days the colonies are 0.6-0.8 mm in diameter |
Liquid |
Growth Parameters | Xylophilus ampelinus |
PHYSIOLOGICAL |
Tropism | chemoorganotroph |
Oxygen | strict aerobe |
pH | |
Temperature | optimum 24 oC range 6-30 oC |
Requirements | Grow is generally slow and poor even under optimum conditions. Best growth on agar containing 1% yeast extract, 2% D-galactose, 2% CaCO3 and 2% agar. Growth occurs on media with L-glutamine but not on calcium lactate (contrast with Xanthomonas) |
Products | nitrates not reduced. H2S formed from cysteine. Does not hydrolyse esculin, starch, casein, arbutin or sodium hippurate indole negative, Acetoin negative |
Enzymes | oxidase negative, catalase positive, urease psotive, lecitinase negative, arginine dihydrolase negative, ornithine decarboxylase negative, lysine decarboxylase negative, lipase negative, geleatinase negative |
Unique features |
ENVIRONMENTAL |
Habitat | isolated from Vitis vinifera |
Lifestyle | |
Pathogenicity | plant pathogens that casue bacterial necrosis and bacterial canker |
Distribution | Isolated from the Mediterranean region and South Africa and similar sypmptoms have been reported in Switzerland, Austria, Bulgaria, the Canarary Islands and Argentina |
Genome | Xylophilus ampelinus |
G+C Mol % |
Reference | Xylophilus ampelinus |
First Citation | Willems et al 1987 IJSB 37:422-430 |
The Prokaryotes | p |
Bergey's Determinative | p 100-101 |
References |