Cross Index	Phenylobacterium immobile
SuperSet	Prokaryote, Eubacteria, Gram-Negative Aerobic/ Microaerophilic Rods and Cocci
Compare	Acetobacter, Acidiphilium, Acidomonas methanolica, Acidothermus cellulolyticus, Acidovorax, Acinetobacter, Afipia, Agrobacterium, Agromonas oligotrophica, Alcaligenes, Alteromonas, Aminobacter, Aquaspirillum, Azomonas, Azorhizobium caulinodans, Azotobacter, Bacteroides, Beijerinckia, Bordetella, Bradyrhizobium japonicum, Brucella, Chromohalobacter marismortui, Chryseomonas luteola, Comamonas, Cupriavidus necator, Deleya, Derxia gummosa, Ensifer adherans, Erythrobacter longus, Flavimonas oryzihabitans, Flavobacterium, Francisella, Frateuria aurantica, Gluconobacter, Halomonas, Hydrogenophaga, Janthinobacterium lividum, Kingella, Lampropedia hyalina, Legionella, Marinobacter hydrocarbonoclasticus, Marinomonas, Mesophilobacter marinus, Methylobacillus glycogenes, Methylobacterium, Methylococcus, Methylomonas, Methylophaga, Methylophilus, Methylovorus glucosotrophus, Moraxella, Morococcus cerebrosus, Neisseriaceae, Neisseria, Oceanospirillum, Ochrobactrum anthropi, Oligella, Paracoccus, Phenylobacterium immobile, Phyllobacterium, Pseudomonas, Psychrobacter immobilis, Rhizobacter daucus, Rhizobium, Rhizomonas suberifaciens, Rochalimaea, Roseobacter, Rugomonas rubra, Serpens, Sinorhizobium, Sphingobacterium, Taylorella equigenitalis, Thermoleophilum, Thermomicrobium, Thermus, Variovorax paradoxus, Volcaniella eurhalina, Weeksella, Wolinella, Xanthobacter, Xanthomonas, Xyella fastidiosa, Xylophilus ampelinus, Zoogloea ramigera
Contrast	Archaea

 

Morphology

Phenylobacterium immobile

CELLULAR

Staining	Gram negative
Morphology	Rods, coccal rods or cocci (0.7-1.0 X 1.0- 2.0 um) that occur singly in paris and in short chains Sometimes clumps occur. Old cultures are pleomorphic with ellipsoids, long rods or long chains connected by filaments
Motility	nonmotile
Specialized structures	no spores, sheaths, prostheecase. Negative in the capsule stain but a fexible capsule is present

Division

COLONIAL

Solid surface

Growth is slow. Colonies are visible (1.2 mmm) after 2-3 weeks. Colonies that are smooth, convex, moist with shiny surfaces, and have an entire edge are easily emuslified in saline. Colonies that are rough and dry are not emuslified in saline. Cultures grown on phenylalanine produce a yellowish green fluorescent pigment

Liquid

In liquid media a greenish-yello non fluorescent pigment is produced

 

Growth Parameters

Phenylobacterium immobile

PHYSIOLOGICAL

Tropism	chemoorganotroph
Oxygen	obligate aerobe
pH	optimum pH 6.8-7.0
Temperature	optimum 29- 30 oC No growth at 4 oC or 37 oC
Requirements	vitamin B12 required as growth factor High nutritional specialization Good growth on chloridazon, antipyrin and L- phenylaline. Slow growth on glutamate, pyruvate, fumarate, succinate and malate. Most sugars, alcohols, amino acids acarboxylic acids and ordinary complex media are not used NH4 + and NO3 - used as the sole sources of nitrogen
Products	do not denitrify. Do not reduce nitrate to nitrite. Gelatin, casein, starch and esculin are ot hydrolysed. no reaction on litmus milk. Methyl red negative. Acetoin not produced. Indole negative. No acid or gas produced from sugars or alcohols.
Enzymes	catalase positive, oxidase weakly positive. urease negative
Unique features	osmotically sensitive. Cells isolated from soil after enrichment with chloridazon, antipyrin or pyramidon

ENVIRONMENTAL

Habitat	soil
Lifestyle
Pathogenicity	not pathogenic for rabbits and rabbits

Distribution

 

Genome

Phenylobacterium immobile

G+C Mol %

 

Reference

Phenylobacterium immobile

First Citation	Makkar Lingens et al 1985 IJSB 35:26-39
The Prokaryotes	p
Bergey's Determinative	p 93

References