Morphology | Brucella |
CELLULAR |
Staining | Gram-negative Do not usually show true bioplar staining |
Morphology | Cocci, coccobacilli or short rods, 0.5-0.7 um in diameter and 0.6-1.5 um in length. Arranged singly and, less frequently, in pairs, short chains or small groups. |
Motility | Nonmotile; do not produce flagella. |
Specialized structures | True capsules are not produced.. Resting stages are not known.. |
Division |
COLONIAL |
Solid surface | Colonies on serum- dextrose agar or other clear medium are transparent, raised, convex, with an entire edge and a smooth, shiny surface. They appear a pale honey colour by transmitted light. Nonsmooth variants of the smooth species occur but there are also stable nonsmooth species with a distinctive host range |
Liquid |
Growth Parameters | Brucella |
PHYSIOLOGICAL |
Tropism | . Chemoorganotrophic.. |
Oxygen | Aerobic, possessing a respiratory type of metabolism and having a cytochrome-based electron transport system with oxygen or nitrate as the terminal electron acceptor |
pH | Optimum pH, 6.6-7.4 |
Temperature | .. Optimum 37'C. Growth occurs between 20 and 40'C |
Requirements | Many strains require
supplementary CO2 for growth, especially on primary
isolation Most strains require complex media containing several amino acids, thiamin, nicotinamide and magnesium ions; some strains may be induced to grow on minimal media containing an ammonium salt as the sole nitrogen source. Growth is improved by serum or blood, but hemin (X-factor) and nicotinamide adenine dinucleotide (NAD: V-factor) are not essential. |
Products | Acid production does not
occur from carbohydrates in conventional media, except
for B. neotomae. Do not produce indole. Do not
liquefy gelatin or inspissated serum. Do not lyse erythrocytes. Do not produce acetyl methyl carbinol (Voges-Proskauer test). The methyl red test is negative. |
Enzymes | Nitrate reductase is produced.. Catalase-positive. Usually oxidase-positive, |
Unique features | Possess characteristic intracellular antigens specific for the genus., |
ENVIRONMENTAL |
Habitat | transmissible to a wide range of animal species including man |
Lifestyle | Intracellular parasites |
Pathogenicity |
Distribution |
Genome | Brucella |
G+C Mol % | 55-58 |
Reference | Brucella |
First citation | Meyer, K.F. and E.B. Shaw (1920) A comparison of the morphological, cultural and biochemical characteristics of B. abortus and B. melitensis. Studies on genus Brucella nov. gen. J. Infect Dis. 27-173-184 |
The Prokaryotes | p |
Bergey's Systematatic | p 377 M. J. Corbel and W. J. Brinely-Morgan |
Bergey's Determinative | p 79 |
References |