Morphology | Acetobacter |
CELLULAR |
Staining | Gram-negative (in a few cases Gram-variable). |
Morphology | Cells ellipsoidal to rod shaped, straight or slightly curved, 0.6-0.8 um by 1.0-4.0 um, occurring singly, in pairs or in chains. Involution forms frequent in some strains; may be spherical, elongated, swollen, club shaped, curved or filamentous |
Motility | Motile or nonmotile; if motile, the Flagella are peritrichous or lateral |
Specialized structures | Endospores are not formed |
Division |
COLONIAL |
Solid surface | Pale colonies; most strains produce no pigments. A minority of strains produce brown water-soluble pigments or show pink colonies due to porphyrins |
Liquid |
Growth Parameters | Acetobacter |
PHYSIOLOGICAL |
Tropism | Chemoorganotrophs |
Oxygen | Obligately aerobic; metabolism is respiratory, never fermentative |
pH | Optimum for growth is 5.4-6.3. |
Temperature | 25-30`C |
Requirements | The best carbon sources for growth are ethanol, glycerol and lactate |
Products | Oxidizes ethanol to acetic acid. Acetate and lactate are oxidized to CO2 and H2O. Neither lactose nor starch is hydrolyzed Acid is formed from n-propanol, n-butanol and D- glucose.. |
Enzymes | Catalase-positive, oxidase-negative. Absence of gelatin liquefaction, indole or H2S formation. |
Unique features |
ENVIRONMENTAL |
Habitat | Acetobacter occurs in flowers, fruits, honey bees, sake, tequila, palm wine, grape wine, cider, beer, South African Bantu Beer, kefir, brewer's yeast, vinegar, beech- wood shavings of vinegar generators, vinegar acetifiers, sugar cane juice, "tea fungus", vegetable tanning liquors, "nata", garden soil and canal water. |
Lifestyle | |
Pathogenicity | Acetobacter causes pink disease in pineapple fruit and rot in apples and pears. |
Distribution |
Genome | Acetobacter |
G+C Mol % | 51-65 |
Reference | Acetobacter |
First citation | Beijerinck,M.W. (1898) Uber die Arten der Essigbakterien. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. II 4:209-216 |
The Prokaryotes | p |
Bergey's Systematatic | p 268 J. DeLey, J. Swings and F. Gossele |
Bergey's Determinative | p71 |
References |