Cross Index	Methylophilus methylotrophus
SuperSet	Prokaryote, Eubacteria, Gram-Negative Aerobic/ Microaerophilic Rods and Cocci
Compare	Acetobacter, Acidiphilium, Acidomonas methanolica, Acidothermus cellulolyticus, Acidovorax, Acinetobacter, Afipia, Agrobacterium, Agromonas oligotrophica, Alcaligenes, Alteromonas, Aminobacter, Aquaspirillum, Azomonas, Azorhizobium caulinodans, Azotobacter, Bacteroides, Beijerinckia, Bordetella, Bradyrhizobium japonicum, Brucella, Chromohalobacter marismortui, Chryseomonas luteola, Comamonas, Cupriavidus necator, Deleya, Derxia gummosa, Ensifer adherans, Erythrobacter longus, Flavimonas oryzihabitans, Flavobacterium, Francisella, Frateuria aurantica, Gluconobacter, Halomonas, Hydrogenophaga, Janthinobacterium lividum, Kingella, Lampropedia hyalina, Legionella, Marinobacter hydrocarbonoclasticus, Marinomonas, Mesophilobacter marinus, Methylobacillus glycogenes, Methylobacterium, Methylococcus, Methylomonas, Methylophaga, Methylophilus methylotrophus , Methylovorus glucosotrophus, Moraxella, Morococcus cerebrosus, Neisseriaceae, Neisseria, Oceanospirillum, Ochrobactrum anthropi, Oligella, Paracoccus, Phenylobacterium immobile, Phyllobacterium, Pseudomonas, Psychrobacter immobilis, Rhizobacter daucus, Rhizobium, Rhizomonas suberifaciens, Rochalimaea, Roseobacter, Rugomonas rubra, Serpens, Sinorhizobium, Sphingobacterium, Taylorella equigenitalis, Thermoleophilum, Thermomicrobium, Thermus, Variovorax paradoxus, Volcaniella eurhalina, Weeksella, Wolinella, Xanthobacter, Xanthomonas, Xyella fastidiosa, Xylophilus ampelinus, Zoogloea ramigera
Contrast	Archaea

 

Morphology

Methylophilus methylotrophus

CELLULAR

Staining	Gram negative but may not take the stain up well
Morphology	straight of slightly curved rods (0.3-0.6 x 0.8-1.5 um) that occur singly or in pairs.
Motility	either nonmotile or motile by single polar flagellum
Specialized structures	no spores, cellular inclusions, sheath, prosthecae or capsules. Some strains produce a slime

Division

COLONIAL

Solid surface

colonies on methanol-mineral salts agar plates after 2 days incuabation at 30- 37 oC are circular (1-2mm) diameter) with an entire edge, convex, translucent to opaque. colonies are greyish whitePyrocyanin and fluorescein are not produced. Extremely poor growth if any on either nutrient or blood agar.No growth on 0.01% potassium tellurite. No growth on 5% NaCl

Liquid

 

Growth Parameters

Methylophilus methylotrophus

PHYSIOLOGICAL

Tropism	chemoorganotroph
Oxygen	aerobe
pH	optimum pH 6.5-7.2
Temperature	optimum 30- 37oC no growth at 4 oC or 45 oC
Requirements	nutritionally non exacting; nitrate and ammonium salts serve as nitrogen sources
Products	very little or no acid is produced from glucose. Methanol is oxidized as the sole carbon and energy source by all strains. A limited range of carbon copounds such as methylamines, formatem glucose, fructose may be used as the sole carbon source. The fatty acid compositionis primarily of the nonhydroxylated straight chain saturated and monounsaturated types with C16:0 and C16:1 predominating. The major isoprenoid quinone components are ubiquinones with eight isoprene units Acetoin may or may not be produced. H2S not produced.
Enzymes	catalase positive, oxidase positive, urease positive. Leucine arylamindase positive, phosphatase production is weak and variable, sulfatase negative, geleatinase negative. DNase negative, RNase negative
Unique features	resistant to penicillin, oleandomycin, sensitive to malidixic acid, streptomycin and several other antibiotics

ENVIRONMENTAL

Habitat	activated sludge, mud, river and pond water.
Lifestyle
Pathogenicity

Distribution

 

Genome

Methylophilus methylotrophus

G+C Mol %

 

Reference

Methylophilus methylotrophus

First Citation	Jenkins et al 1987 IJSB 37: 446-448
The Prokaryotes	p
Bergey's Determinative	p 89-90

References