| Morphology | Hemophilus |
| CELLULAR |
| Staining | Gram-negative |
| Morphology | Minute to medium-sized coccobacilli or rods, generally less than 1 um in width and variable in length, sometimes forming threads or filaments and showing marked pleomorphism.. |
| Motility | Nonmotile |
| Specialized structures |
| Division |
| COLONIAL |
| Solid surface |
| Liquid |
| Growth Parameters | Hemophilus |
| PHYSIOLOGICAL |
| Tropism | Chemoorganotrophic |
| Oxygen | Aerobic or facultatively anaerobic |
| pH | |
| Temperature | Optimum temperature, 35`37`C |
| Requirements | Require preformed growth factors present in blood, particularly X factor (protoporphyrin IX or protoheme) and/or V factor (nicotinamide adenine dinucleotide (NAD) or NAD phosphate (NADP). Even after specific growth factors have been provided, growth is best on complex media |
| Products | Nitrates are reduced to, or beyond, nitrites All species can attack carbohydrates fermentatively, yielding acetic, lactic, and succinic acids as end products in glucose broth |
| Enzymes | Oxidase and catalase reactions vary among strains |
| Unique features |
| ENVIRONMENTAL |
| Habitat | mucous membranes of man and a variety of animals species. |
| Lifestyle | obligate parasites |
| Pathogenicity |
| Distribution |
| Genome | Hemophilus |
| G+C Mol % | 37-44 (Tm) |
| Reference | Hemophilus |
| First citation | Winslow,C.-E.A.,J. Broadhurst,R.E. Buchanan, C. Krumwiede Jr., L.A.Rogers and G.H. Smith (1917) The families and genera of the bacteria .Preliminary report of the Committee of the Society of American Bacteriologists on characterization and classification of bacterial types. J. Bacteriol 2:506-566 |
| The Prokaryotes | p |
| Bergey's Systematatic | p 558 M. Kilian and E. L. Biberstein |
| Bergey's Determinative | p 195 |
| References |