Leukocyte Migration & Inflammation

They say that what you don’t know won’t hurt you
Some of us haven’t felt a twitch of pain in years
Fletcher Knebel

Who Am I? Trained as a zoologist, I coined the term phagocyte after observing the response of starfish larvae to rose thorns. I late showed that white blood cells are phagocytic

RESOURCES

Lymphocyte migration

Lymphocyte migration as shown in "The Anatomy of a Splinter" at the Cell Alive! site.

URL --> http://www.cellsalive.com/ouch.htm

Chemotaxis

Chemotaxis as shown in "The Anatomy of a Splinter" at the Cell Alive! site.

URL --> http://www.cellsalive.com/chemotx.htm

Chemokines

PeproTech, Inc., commercial site, company that says its mission is the "development and manufacture of recombinant cytokines for life science research" Provides text on chemokines; some history and description of chemokines. Contains bibliogrphy of 11 research articles that are relevant to chemokines.

URL --> http://www.peprotech.com/DOCS/cytobull.htm

Chemokines - an article

An article in Life Science News, a publication of Amersham Life Science, Issue 19 "Aiding the Study of Chemokines" by Dave J. Bryant and Arvind C. Patel. Provides references to 17 research articles that are relevant to chemokines.

URL --> http://www.amersham.co.uk/life/lsn/19/pages/p21-full.htm

ASSIGNED READING

CHAPTER 15: pp 357-378

OUTLINE/SUMMARY

1. Lymphocyte Recirculation
2. Cell Adhesion Molecules
   • Selectin family
   • Mucin-like family
   • Integrin family
   • Immunoglobulin superfamily
3. Neutrophil Extravasation
4. Lymphocyte Extravasation
   • High endothelial venules
   • Lymphocyte homing
   • naive lymphocytes
   • effector lymphocytes
   • memory lymphocytes
   • Adhesion molecule interactions
5. Mediatiors of Inflammation
   • Chemokines
   • Plasma enzyme mediators
   • kinins
   • clotting system
   • fibrinolytic system
   • complement
6. Lipid inflammatory mediators
7. Cytokine inflammatory mediators
8. Inflammatory Process
9. Role of neutrophils
10. Acute inflammatory response
    • localized response
    • systemic acute phase response
11. Chronic inflammatory response
    • role of IFNs
    • chronic inflammatory diseases
12. Anti-inflammatory Agents
    • Reduction of Leukocyte extravasation
    • corticostroids
    • Nonsteroidal anti inflammatories

PERFORMANCE OBJECTIVES

DEFINE THE FOLLOWING KEY TERMS:

ON COMPLETION OF THIS SECTION THE STUDENT SHOULD BE ABLE TO:

1. Describe lymphocyte recirculation
2. Describe four classes of cell adhesion molecules
3. Describe four sequential but overlapping steps in neutrophil extravasation.
4. Discuss the role of G proteins in neutrophil extravasation.
5. Describe the location, structure and function of High endothelial venules
6. Distinguish between neutrophilic and lymphocytic extravasation
7. Construct a table to compare the C-C subgroup of chemokines with the C-X-C subgroup of chemokines
8. Describe the inflammatory process.
9. Compare the localized acute inflammatory response with the systemic acute phase response.
10. Draw a diagram to give an overview of the cells and mediators involved in a local acute inflammatory response [371]
11. Draw a diagram to give an overview of the cells and mediators involved in a systemic acute inflammatory response [372]
12. Describe the chronic inflmmatroy response [373]
13. List 5 chronic inflammatory diseases associated with HEV [375]
14. Discuss the use of antiinflammatory agents to reduce long term inflammatory responses in infections, transplants and burns.

SHORT ANSWER QUESTIONS