| Technique | CYANIDE ,(/,)/,( |
| Principle | |
| Cautions | POTASSIUM
CYANIDE CAUTION EXTREMELY TOXIC USE GREAT CARE!!! ADD FERROUS SULPHATE AND 40% POTASSIUM HYDROXIDE TO DESTROY THE CYANIDE BEFORE TUBES OR BOTTLES ARE PUT IN THE AUTOCLAVE THIS TEST IS NOT RECOMENDED FOR STUDENT USE |
| Method | Inoculate KCN broth with
one loopful of an overnight broth culture. TIGHTLY screw down the cap of the bottle and incubate for up to 48 hours. |
| Results | Turbidity which indicated growth constitutes a positive reaction |
| Positive control | Klebsiella aerogenes |
| Negative control | Escherichia coli |
| Reagents | |
| Reference |