| Technique | TWEEN HYDROLYSIS TEST ,(/,)/,( | |||||||||
| Principle | Some mycobacteria produce a lipase that hydrolyses Tween 80 (poly oxyethylene sorbitan monooleate) into oleic acid and polyoxyethylated sorbitol; | |||||||||
| Cautions | ||||||||||
| Method | a colour change from the straw yellow to pink can be observed | |||||||||
| Results | ||||||||||
| Positive control | ||||||||||
| Negative control | ||||||||||
| Reagents | 
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| Reference |