Technique
TWEEN HYDROLYSIS TEST
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Principle Some mycobacteria produce a lipase that hydrolyses Tween 80 (poly oxyethylene sorbitan monooleate) into oleic acid and polyoxyethylated sorbitol;
Cautions  
Method a colour change from the straw yellow to pink can be observed
Results  
Positive control  
Negative control  
Reagents
Substrate Tween 80 0.5 ml
Neutral Red 0.1% aqueous 2.0 ml
Phosphate buffer 0.067 Molar, pH 7 100 ml
Reference