Stain solutions

Acid Alcohol

Concentrated hydrochloric acid 3 ml

Ethanol (95%) 97 ml

Albert’s Stain Solution:

Malachite green 0.2 gm;

Toluidine Blue 0.15 gm;

95% ethanol 2 ml;

Glacial acetic acid 1 ml;

Distilled water 100 ml.

Dissolve the dyes in the ethanol. Mix the acid with the water and add to the dye solution.

Let stand for 24 hours and then filter.

Ammoniacal silver nitrate

Silver nitrate 5 g

Distilled water 100 ml

Take 90 ml of the above solution and add strong ammonia solution drop by drop until the precipitate that forms just dissolves

Then add the rest of the silver nitrate solution drop by drop until the reagent remains slightly turbid even after shaking

Protect from the light

Stable for several weeks

Carbol Fuchsin

Solution A

Basic fuchsin 10 g

Ethanol (95%) 100 ml

Mix and dissolve and keep at 37 C overnight in a stoppered bottle

Solution B

Phenol 5 g

Distilled water 100 ml

Mix and dissolve

For use add 10 ml of solution A into 100 ml of solution B and mix

Crystal Violet Solution

Dienes Stain

Methylene blue 2.50 gm

Azure II 1.25 gm

Maltose 10.00 gm

Sodium carbonate 0.25 gm

Distilled water 100 ml

Kirkpartick’s Fixative

Absolute ethanol 60 ml

Chloroform 30 ml

Formaldehyde (40%) soln 10 ml

Loeffler’s Methylene Blue

Gram’s (Lugol’s) Iodine Solution

Plimmer’s Mordant

Iron Tannate Mordant

Giemsa Stain solution

Gram alcohol solution

Ethanol

water

Malachite Green Solution

Muir’s Mordant

Neisser’s stain

For use, mix 20 ml of solution A and 10 ml of solution B

Neisser’sSolution A:

Methylene blue 0.1 gm;

95% ethanol 5 ml;

Glacial acetic acid 5 ml

Distilled water 100 ml.

Dissolve the dye in the water and add the acid and ethanol

Neisser’s Solution B:

Crystal violet 0.33 gm;

95% ethanol 3.3 ml

Distilled water 100 ml.

Dissolve the dye in the ethanol- water mixture

Safranin Solution

Safranin 0.5 g

Water 100 ml

Sudan Black B Solution

Sudan Black B 0.3 gm

95% ethanol 75 ml

Distilled water 25 ml

Dissolve the Sudan Black B in 95% ethanol then add water and mix thoroughly