| Technique | MOTILITY MEDIUM ,(/,)/,( |
| Principle | Motile organisms will disperse in a medium that contain SMALL amounts of agar. This can be seen as a fuzzy outline in such a "sloppy" agar deep. Those organisms which reduce tetrazolium salts to coloured end products can be seen even more clearly.. |
| Cautions | TEMPERATURE Most motile microbes are motile at lower temperatures (15-25 degrees C) and may not be motile at the optimal temperature for growth. |
| Method | A deep of tetrazolium motility medium is inoculated by stabbing down the centre carefully. Sometimes a small glass Craigie tube is embedded in the motility medium so that the upper end of the tube is above the top of the medium. The inner tube is innoculate with the microbe and the tube is incubated for24 -48 hours, after which motile microbes can be isolated from the outer tube. |
| Results | After 24-48 hours incubation fuzziness and/or a red precipitate away from the s tab line indicates motility |
| Positive control | |
| Negative control | |
| Reagents | |
| Reference |