|Principle||For metachromatic granules (volutin granules) make a smear from a culture grown on Loeffler serum|
|Method||See Neisser's Stain
See Albert's Stain
|Technique||METACHROMATIC GRANULES Neisser's Stain|
|Method||Stain with Neisser's stain for 10
Rinse rapidly with water.
Stain with 0.2% Bismarck brown or
0.4% chrysoidin for 30 seconds.
Wash rapidly in water, drain or blot dry.
|Results||Cytoplasm appears light brown. Granules blue-black.|
|Reagents||Neissers stain solution
For use, mix 20 ml of solution A and 10 ml of solution B
Dissolve the dye in the water and add the acid and ethanol
Dissolve the dye in the ethanol- water mixture
|Technique||METACHROMATIC GRANULES Albert's Stain|
|Method||1: Stain with Albert's stain fo 3-5
2: Rinse with water
3: Blot dry.
3: Stain with Lugol's iodine solution for 1 minute.
4: Rinse with water
5: Drain or blot to dry.
|Results||Cytoplasm appears light green, granules blue-black|
|Reagents||Alberts Stain Solution:
Malachite green 0.2 gm;
Toluidine Blue 0.15 gm;
95% ethanol 2 ml;
glacial acetic acid 1 ml;
distilled water 100 ml.
Dissolve the dyes in the ethanol. Mix the acid with the water and add to the dye solution. Let stand for 24 hours and then filter.
Lugols Iodine Solution
|Reference||H. Albert 1921 J. Am. Med Ass 76:240|