| Technique | GLUCONATE ,(/,)/,( |
| Principle | Gluconate can be converted to 2-ketogluconate which can be detected by the appearance of a reducing substance in the medium. The test is useful in identifying Pseudomonas aeruginosa, Enterobacter species and those Klebsiella with IMViC reaction --++. |
| Cautions | |
| Method | Inoculate gluconate broth and incubate for 2 days. Add 1 ml of Benedict's solution, mix and boil for 10 min. Clintest tablets (Ames Co) can replace Benedict's solution |
| Results | The formation of a brown, orange or tan preciptate is a positive reaction |
| Positive control | Klebsiella aerogenes |
| Negative control | Escherichia coli |
| Reagents | |
| Reference |