| Technique | GIEMSA STAIN for CHLAMYDIAE ,(/,)/,( |
| Principle | |
| Cautions | Stain must be freshly prepared |
| Method | 1: Prepare smears 2: Air dry 3: Fix smears in absolute methanol for 5 minutes 4: Air dry. 5: Flood smear with diluted stain 6: Leave for 1 hour. 7: Rinse in 95% ethanol 8: Air dry. 9: Examine with the oil immersion lens |
| Results | Elementary bodies will stain purple,
Initial bodies appear blue. The cytoplasm stains gray, The cell nucleus is pink. |
| Positive control | |
| Negative control | |
| Reagents | Giemsa Stain Solution |
| Reference |