|Technique||DIENES STAIN for Mycoplasma|
|Method||1: Rinse a coverslip with
alcohol to remove any grease.
2: Let the coverslip dry
3: Spread a drop of Dienes stain on the cover slip
4: Let the stain dry.
5: Press the stained face of the cover slip against the suspected Mycoplasma colonies
6: Examine microscopically using the low power and high-dry lenses
|Results||Mycoplasma colonies have a distinctive dense blue stained center and light blue peripheries|
|Reagents||Dienes Stain solution
Methylene blue 2.50 gm
Azure II 1.25 gm
Maltose 10.00 gm
Sodium carbonate 0.25 gm
Distilled water 100 ml
|Reference||Dienes & Weinberger 1951 Bact Rev 15:245)|