| Technique | DIENES STAIN for Mycoplasma |
| Principle | |
| Cautions | |
| Method | 1: Rinse a coverslip with
alcohol to remove any grease. 2: Let the coverslip dry 3: Spread a drop of Dienes stain on the cover slip 4: Let the stain dry. 5: Press the stained face of the cover slip against the suspected Mycoplasma colonies 6: Examine microscopically using the low power and high-dry lenses |
| Results | Mycoplasma colonies have a distinctive dense blue stained center and light blue peripheries |
| Positive control | |
| Negative control | |
| Reagents | Dienes Stain solution Methylene blue 2.50 gm Azure II 1.25 gm Maltose 10.00 gm Sodium carbonate 0.25 gm Distilled water 100 ml |
| Reference | Dienes & Weinberger 1951 Bact Rev 15:245) |