| Technique | DNASE ,(/,)/,( |
| Principle | DNAse can be used as differential test for strains of Staphylococci |
| Cautions | |
| Method | DNA plates contain polymeric DNA which can be hydrolysed to nucleic acids by the DNAse. After 24 hours incubation at 37 degrees C, the inoculated plate is flooded with 1 N hydrochloric acid, which will precipitate undigested DNA but will leave a clear zone around the DNAse positive strains. Alternatively, the plate may be flooded with 0.1% toluidine blue. A pink colour is produced around the growth of DNAse producers. The test is useful for the study of Bacillus, Staphylococcus, Streptococcus and Pseudomonas. |
| Results | |
| Positive control | |
| Negative control | |
| Reagents | |
| Reference |