| Technique | COAGULASE ,(/,)/,( |
| Principle | Some strains of Staphylococci produce agents called Staphylocoagulase that promote the clotting of plasma. Staphylocoagulase is made up of free and bound coagulase |
| Cautions | Do not use plasma that contains EDTA
The type of plasma may affect the result. The citrate in citrated plasma may be removed by some citrate utilizing bacteria which may cause a clot to form after prolonged incubation. Heparin can be added to citrated plasma to prevent this problem. Production of large amounts of fibrinolysin wil dissolve the clot |
| Method | . Slide methods detect just bound coagulase while tube methods detect both forms. |
| Results | |
| Positive control | |
| Negative control | |
| Reagents | |
| Reference |