| Technique | CAMP ,(/,)/,( |
| Principle | Group B streptococci produce an extracellular factor called the CAMP factor that enhances the hemolytic activity of staphylococcal beta lysin on sheep erythrocytes. When the CAMP factor and Staphylococcal beta lysin overlap in a sheep bl ood agar plate, the Beta hemolytic reaction is accentuated. |
| Cautions | Incubation of the plate under carbon dioxide may result in false positive reactions with Streptococcus pyogenes |
| Method | A single streak of Staphylococcus aureus that produces beta lysin is made on a sheep blood agar plate; a streak of the streptococcal test culture is made perpendicular to the S. aureus streak but WITHOUT TOUCHING IT. The plate is incubated in air at 35-37 C overnight. The zone of enhanced hemolysis appears in the shape of an arrowhead at the junction of the two streak lines. |
| Results | |
| Positive control | |
| Negative control | |
| Reagents | |
| Reference |