Microbial Genetics

 

Readings Presentations Key Terms Questions Multiple Choice Quizzes Updated 13/02/01

Children have to be educated, but they have also to be left to educate themselves
Ernest Dimnet

Who am I? I demonstrated that antigens, when properly presented, induced some lymphocytes to develop into plasma cells. I was the foirts to formally demonstrate the one cell- one antibody rule

 

Readings Microbial Genetics
Baron
Medical Microbiology
Chap 5: genetics		 Brock 9th Ed
Biology of Microorganisms
Chapt 9 Microbial Genetics
Chapt 7
Regulation of Gene Expression
Tortora:
Microbiology An Introduction
Chapt 8 Microbial Genetics		 Jacquelyn Black 4th Ed
Microbiology:
Principles & Explorations
Chapt 7 Genetics I
Chapt 8 Genetics II
Presentation Slides Web Animation Download
Protein Synthesis A  
Protein Synthesis B  
Protein Synthesis C  
DNA  
Genes 1  
Genes 2  
Bacterial Genetics  

Define the Following Key Terms
Genetics chromosome Genes base pairs
genetic code phenotype replication fork leading strand
lagging strand RNA primer DNA ligase mRNA
promotor terminator translation codon
nonsense codon tRNA repression repressor
induction inducer operon operator
corepressor point mutation missense mutation nonsense mutation
frameshift mutation F factor mutagen transposon
transduction R factor transformation conjugation
plasmid      

QUESTIONS

  1. Describe how DNA serves as genetic information
  2. Describe the process of DNA replication through the use of diagrams
  3. Summarize six (6) steps in the events at the DNA replication fork
  4. Describe protein synthesis, including transcription, RNA processing and translation
  5. Explain the regulation of gene expression in bacteria by induction, repression, and catabolic repression
  6. Classify mutations by type and describe how mutations are prevented or repaired.
  7. Compare the mechanisms of genetic recombination in bacteria
  8. Describe the functions of plasmids and transposons
  9. Describe translation and be sure to include the following terms; reibosome, rRNA, amino acid activation,tRNA, anticodon and codon.
  10. Define plasmids and explain the relationship between F factors and conjugation
  11. Base analogs and ionizing radiation are used in the treatment of cancer. These mutatgens can cause cancer, so how do you suppose they are used to treat the disease?
  12. Replication of the E. coli chromosome takes 40-45 min but the organism has a generation time of 26 minutes. How does the cell have time to make complete chromosomes for each daughter cell? for each granddaughter cell?
  13. A dentist experienced symptoms of HIV infections in late 1986 and was diagnosed with AIDS in 1987. He closed his practice in 1989. All of the dentist's employees, including the hygienists tested negative for HIV antibodies. The dentist wrote an open letter to his former patients, which prompted 591 people to be tested for HIV anti-bodies; seven tested positive, The virus that causes AIDS, was isolated from these seven individuals and the amino acid sequences for the viral coat were determined. Of the three amino acid sequences shown below, which one was NOT contracted from the dentist? Why can these amino acid sequences be used to identifiy the source of a virus?
Patient Viral Amino Acid Sequence
A Asn Gln Thr Ala Ala Ser Lys Asn Ile Asp Ala Glu Leu
B Asn Leu His Ser Asp Lys Ile Asn Ile Ile Leu Gln Leu
C Asn Gln Thr Ala Asp ... Ser Ile Val Ile Asp Ala Cys
  1. Chloroquine, erythromycin and acylovir are sued to treat microbial infections. Chloroquine acts by fitting between base pairs in the DNA molecule. erythromycin acts by binds in front of the A site on the 50S subunit of a ribosome. Acylovir is a guanine analog.