Growth of Prokaryotes

 

Readings Presentations Key Terms Questions Multiple Choice Quizzes Updated 13/02/01

"Predigested food" should be inscribed over every hall of learning as a warning to all
who do not wish to lose their own personalities and their original sense of judgement
Emma Goldman

Who Am I? As an endocrinologist I developed the technique of radioimmunoassay for which I received the Noble prize in medicine in 1977

 

Readings Growth of Prokaryotes
Baron
Medical Microbiology
Chap 4: Bacterial Metabolism
 Brock 9th Ed
Biology of Microorganisms
Chap 5 Microbial Growth
Tortora:
Microbiology An Introduction
Chapter 6
Growth of Prokaryotes		 Jacquelyn Black 4th Ed
Microbiology:
Principles & Explorations
Chap 6
Growth and Culturing of Bacteria
Presentation Slides Web Animation Download
Nutrients  
Growth  
Cell Counts  
 
KEY TERMS
Psychrophile psychrotroph extreme halophiles superoxide free radicals
Mesophile hyperthermophiles halophiles superoxide dimutase
Thermophile extreme thermophiles obligate halophiles catalase
minimum growth temperature acidophiles facultative halophiles peroxide ion
optimum growth temperature plasmolysis nitrogen fixation peroxidase
maximum growth temperature obligate aerobes trace elementes organic growth factors
obligate anaerobes facultative anaerobes aerototlerant anerobes microaerophiles
culture medium sterile agar turbidity
chemically defined media complex media selective media differential media
enrichment media colony streak plate method binary fission
budding generation time lag phase log phase
exponential growth stationary phase death phase plate counts
serial dilution pour plates spread plates  

SHORT ANSWER QUESTIONS

  1. List the chemical requirements for microbial growth
  2. List the physical requirements for microbial growth
  3. Classify microbes into five groups on the basis of preferred temperature range
  4. Explain the importance of osmotic pressure to microbial growth
  5. Identify why the pH of culture media is controlled
  6. Identify how the pH of culture media is controlled
  7. Describe how pure cultures can be isolated using the dilution plate method
  8. Describe bacterial growth, including binary fission
  9. Describe the four steps in binary fission
  10. Describe four direct methods of measuring cell growth
  11. Describe how to enumerate bacteria by the serial dilution method
  12. Describe the structure and use of a Petroff-Hausser cell counter
  13. Compare the phases of microbial growth and describe their relation to generation time
  14. Describe how to enumerate bacteria by filtration
  15. Describe how to enumerate bacteria by the most probable number method
  16. Differentiate between direct and indirect methods of measuring cell growth
  17. Explain three indirect methods of measuring cell growth
  18. A pastry chef accidently inoculated a cream donut with six cells of Staphylococcus aureus. If Staphylococcus aureus has a generation time of 60 minutes, how many cells would be in the cream pie after 7 hours (Note the generation time and the incubation time could be modfyied on the actual exam question so learn the principle rather than just memorizing the answer)
  19. Why can high concentrations of salt or sugar be used to preserve food
  20. Draw a typical bacterial growth curve, label and define each of the four phases
  21. Draw the following growth curves for E. coli, a mesophile, starting with 100 cells with a generation time of 30 min at 35 C. This question can be modified to use a pycrohiles, a psycrotroph a thermophile or a thermotroph and the temperatures and incubation periods can be also changed. Learn the principle not just the answers