Observing Microorganisms Through a Microscope

If there is anything that we wish to change in the child, we should first examine it and see whether it is not something that could be better changed in ourselves.Carl Jung
Everybody likes a compliment Abraham Lincoln	This is Uncle Staphylococcus, no its Aunt Streptococcus, Criminey! its an air bubble

Who Am I? Trained as a zoologist, I coined the term phagocyte after observing the response of starfish larvae to rose thorns. I late showed that white blood cells are phagocytic

	Readings: Observing Microorganisms Through the Microscope
	Baron Medical Microbiology Chapter 2 Structure		Brock 9th Ed Biology of Microorganisms Chapt 3 Cell Biology
	Tortora: Microbiology An Introduction Chap 3 Observing Microorganisms Through the Microscope		Jacquelyn Black 4th Ed Microbiology: Principles & Explorations Chap 3 Microscopy and Staining
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Microscopy

OUTLINE/SUMMARY

Units of Measurement

• The standard unit of measurement is the meter(m)
• Microbes are measured in micrometers (10-6 m) and nanometers( 10 -9 m)

Microscopy

• Compound light Microscope
• Darkfield Microscope
• Phase contrast Microscope
• Differential Interference Contrast Microscope
• Fluorescent Microscope
• Confocal Microscope
• Transmission Electron Microscope
• Scanning Electron Microscope

Preparation of Specimens

• Preparing smears for staining
• Simple Stains
• Differential Stains

SHORT ANSWER QUESTIONS

1. Label the parts of a microscope
2. Describe the uses and features of each of the following types of light microscopes

• Brightfield
• Dark field
• Phase contrast
• Differential Interference Contrast
• Fluorescent
• Confocal

3. Describe the features scanning and transmission electron microscopes
4. Identify the type of microscope that was used from the type of micrograph that is produced